Biosynthesis of Poly(3-Hydroxybutyrate-co‑3-Hydroxyhexanoate) with a High 3-Hydroxyhexanoate Fraction and Low Molecular Weight for Polymer Blending
نویسندگان
چکیده
Polyhydroxyalkanoates (PHAs) are aliphatic polyesters that biosynthesized and accumulate in bacterial cells. Among PHAs, poly(3-hydroxybutyrate-co‑3-hydroxyhexanoate)s [P(3HB‑co‑3HHx)s] known to be practical PHA copolymers with an appropriate degree of flexibility. In the biosynthesis PHA, ethanol induces chain transfer (CT) reaction, resulting low-molecular-weight PHA. this study, P(3HB‑co‑3HHx)s were using recombinant Escherichia coli by feeding fatty acid(s) as 3HHx precursor CT agent, respectively, obtain polymers high fractions low molecular weights. Two P(3HB‑co‑3HHx)s, whose 49 86 mol% weight average weights 1.5×104 2.8×104, blended P(3HB‑co‑5 3HHx) (PHBH5) base material. The blending properties investigated explore how thermal can modified. Thermal analysis differential scanning calorimetry (DSC) showed blends prepared solution casting obviously immiscible when 30 wt.% or more polymer was added, even if its low. polymers, a lower crystallization effect compared neat PHBH5 observed without changing melting temperature. However, promoted material under specific conditions. Thus, is simple method for producing materials altered microstructural
منابع مشابه
Cloning and analysis of the poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) biosynthesis genes of Aeromonas caviae.
A 5.0-kbp EcoRV-EcoRI restriction fragment was cloned and analyzed from genomic DNA of Aeromonas caviae, a bacterium producing a copolyester of (R)-3-hydroxybutyrate (3HB) and (R)-3-hydroxyhexanoate (3HHx) [P(3HB-co-3HHx)] from alkanoic acids or oils. The nucleotide sequence of this region showed a 1,782-bp poly (3-hydroxyalkanoate) (PHA) synthase gene (phaC(Ac) [i.e., the phaC gene from A. cav...
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In support of programs to identify polyhydroxyalkanoates with improved materials properties, we report on our efforts to characterize the mechanical and thermal properties of copolyesters of 3-hydroxybutyrate (3HB) and 3-hydroxyhexanoate (3HHx). The copolyesters, having molar fraction of 3HHx ranging from 2.5 to 35 mol % and average molecular weights ranging from 1.15 x 10(5) to 6.65 x 10(5), w...
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Reduced downstream costs, together with high purity recovery of polyhydroxyalkanoate (PHA), will accelerate the commercialization of high quality PHA-based products. In this work, a process was designed for effective recovery of the copolymer poly(hydroxybutyrate-co-hydroxyhexanoate) (P(HB-co-HHx)) containing high levels of HHx (>15 mol%) from Ralstonia eutropha biomass using non-halogenated so...
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ژورنال
عنوان ژورنال: ?????? ?????????? ???????????? ????????????
سال: 2021
ISSN: ['2313-6049', '1998-2836']
DOI: https://doi.org/10.17516/1997-1389-0363